Medium 199 was specifically designed for culturing non-transformed cells. It is extensively used in virology, vaccine production, and for in vitro culture of primary explants from mouse pancreatic epithelial tissue.
Medium 199, when formulated with Earles salts, includes L-Glutamine and 1.25g/L of sodium bicarbonate.
Biosera advises adding 10% fetal bovine serum to the medium.