Hanks’ Balanced Salt Solution (HBSS) is designed to maintain mammalian cells by providing a chemically defined, balanced salt environment that preserves the structural and physiological integrity of cells in vitro. Hanks’ salts are optimized for maintaining cells under ambient (non-CO2) atmospheric conditions.
HBSS without calcium and magnesium is often used to wash and resuspend cells during the dissociation process, as the presence of these ions can inhibit enzymatic activity (e.g., trypsin).
This formulation contains no sodium bicarbonate or phenol red.
