Iscove’s Modified Dulbecco’s Medium (IMDM) is an enhanced version of Dulbecco’s Modified Eagle’s Medium (DMEM), enriched with selenium, extra amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate in place of ferric nitrate.
IMDM is suitable for supporting murine B lymphocytes, hemopoietic tissues from bone marrow, B cells activated by lipopolysaccharide, T lymphocytes, and various hybrid cell types.
This medium’s formulation excludes sodium bicarbonate and includes L-Glutamine and 25mM HEPES.