Leibovitz L-15 Medium was originally developed for use in carbon dioxide (CO2) free systems without sodium bicarbonate. To help maintain physiological pH control, this medium is buffered by its complement of salts, free base amino acids, and galactose substituted for glucose.
When properly supplemented, Leibovitz L-15 Medium supports established cell lines, such as Hep-2, L929, MRC-5, and LLC-MK2, as well as primary explants of embryonic and adult human tissue. Many viruses have also been successfully cultivated in this medium.
Its formulation does not contain L-Glutamine.
