RPMI-1640 medium was developed by Moore and colleagues at Roswell Park Memorial Institute in 1966 for cultivating human leukemic cells in monolayer or suspension.
RPMI-1640 is a versatile medium with numerous applications for a broad range of mammalian cell lines, including the Jurkat cell line, hybridoma cells, human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes.
The formulation is sodium bicarbonate-free and includes L-Glutamine.